「資料集・リンク」
「専門家向けデータベース」
Atlas of Medaka Gonadal Histology
by MOE and Chemicals Evaluation and Research Institute, Japan (CERI)
INDEX
- Top page of Atlas of Medaka Gonadal Histology
- About Medaka (Oryzias latipes)
- Normal Development and Gonadal Histology in Medaka
- Presentation / Measurement / Breeding Condition
- Normal development of the gonad
- GSI, HSI and VTG levels from 35 to 100-dph
- Atlas of Gonadal Histopathology in Medaka Exposed to Endocrine Disruptors
Normal Development and Gonadal Histology in Medaka
Presentation
We present basic data on normal development of testis and ovary as well as growth, secondary sexual characteristics and vitellogenin synthesis
Measurement
- Gonadal histology
- Growth (total length and body weight)
- Secondary sexual characteristics (after 35 dph)
- Hepatic vitellogenin concentration (VTG) (after 35 dph)
- Gonadosomatic index (GSI) (after 35 dph)
- Hepatosomatic index (HIS) (after 35 dph)
Outline of the study
Breeding conditions
| Species | Medaka (Oryzias latipes) |
| Breeding system | Flow-through |
| Water temperature | 24±1℃ |
| Breeding water | dechlorinated tap water |
| Photoperiod | 16h light, 8h dark |
| Test chamber size | 2.5L |
| Water volume | 1.8L |
| Volume exchange of water | 12 times a day |
| Number of fish per test vessel | 15(15 fish ×9 vessels) |
| Food | newly hatched Artemia nauplii(<24h) |