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[F-3.5 Production of male and female individuals derived from a single fertilized egg]

[Contact Person]

Naito Mitsuru
Head of Genetic Engineering Laboratory
Department of Animal Breeding and Genetics
National Institute of Animal Industry
Ministry of Agriculture, Forestry and Fisheries
Tsukuba Norindanchi P.O. Box 5, Ibaraki 305-0901, Japan
Tel:+81-298-38-8622 Fax:+81-298-38-8606
E-mail:mnaito@niai.affrc.go.jp

[Total Budget for FY1997]

3,011,000 Yen

[Abstract]

In order to develop a novel technique for proliferating endangered animal species, a study was carried out to produce male and female individuals derived from primordial germ cells obtained from a single fertilized egg via germline chimeras. Sexing of donor primordial germ cells and recipient embryos were performed by polymerase chain reaction analysis for detecting the W-chromosome specific DNA sequence in chicken. Primordial germ cells obtained from a single embryo were divided into two parts, and a half of them was transferred into male recipient embryo and the other half was transferred into female recipient embryo. The manipulated embryos were cultured in surrogate eggshells until hatching. So far, 314 embryos were manipulated and obtained 83(26.4%) chicks. Five pairs of chicks obtained by transferring primordial germ cells derived from the single embryos. Mating experiment for examining the germline chimerism of the putative chimeric chickens is going to be performed when they reach sexual maturation.

[Key Words]

Primordial germ cell, Germline chimera, sexing, blastodermal cell