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[F-3.4 Study on the dynamics and cell biology of circulating primordial germ cells in early chick embryos]

[Contact Person]

Atsushi Tajima
Associate professor
Institute of agriculture ad Forestry
University of Tsukuba
Tsukuba, Ibaraki 305, Japan
Tel:+81-298-53-2546 Fax:+81-298-53-6205
E-mail:axt22@psu.edu

[Total Budget for FY1996-FY1998]

9,731,000 Yen (FY1997;1,988,000 Yen)

[Abstract]

We conducted a series of experiments assuming that production of the germline chimeras by collecting, conserving and transferring primordial germ cells (PGCs) would fulfill our goal. In the present study, 8 experiments were performed, those are 1) change in the PGC concentration during early embryonic development, 2) effect of storage on the PGC concentration, 3) effects of female on the concentration of PGCs, 4) change on the concentration of circulating PGCs within an embryo, 5) total number of circulating PGCs in an embryo, 6) a new method of collecting circulating PGCs, and 7) production of germ-line chimeras by transferring cryopreserved gonadal PGCs.

[Key Words]

avian, primordial gem cells, cell number, filtration.