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[F-3.2.2 Development of an experimental system for the evaluation of manipulated primordial germ cells for the ability to develop into functional gametes.]

[Contact Person]

Michiharu Sakurai
Head
Laboratory of Immunobiology
National Institute of Animal Health
Ministry of Agriculture, Forestry and Fisheries
3-1-1 Kannondai, Tukuba-shi, Ibaraki 305, Japan
Tel:+81-298-38-7790 Fax:+81-298-38-7880
E-mail:yukari@niah.affrc.go.jp

[Total Budget for FY1996-1997]

4,755,000 Yen (FY1997;2,700,000 Yen)

[Abstract]

Transplantation of primordial germ cells (PGCs) of endangered avian species into embryos of other birds is expected to be a feasible manner to preserve and multiply the endangered wild life. The final goal of this study was to develop an experimental system to test the quail PGCs which had been manipulated in vitro for the ability to differentiate into functional gametes and thus to give rise to progeny. Toward this goal, the conditions for efficient cryopreservation of quail PGCs were established. And a method for culture of the quail embryos into which exogenous PGCs were injected was developed. By using these techniques, PGCs obtained from the gonadal anlage of wild-type and Fl (AMRP x SBPN) embryos were stored in liquid nitrogen for up to 5 months, and transferred to quail embryos of the other strain. Upon mating of the chimeras with Fl quail, the progenies derived from injected PGCs were obtained, indicating the cryopreserved PGCs retained the ability to differentiate!! into functional gametes. This result also indicates that the methods developed in this study provide a feasible experimental system for the evaluation of the ability of quail PGCs to become functional gametes.

[Key Words]

avian, primordial germ cells, endangered wildlife, cryopreservation, progeny test