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[F-3.1 Studies on the production of interspecific chimera using primordial germ cells.]

[Contact Person]

Takashi Kuwana
Head
Pathology Section
National Institute for Minamata Disease
Environment Agency
Hama 4058-18, Minamata, Kumamoto, 867-0008, Japan
Tel:+81-966-63-3111 Fax:+81-966-61-1145
E-mail:kuwana@fsinet.or.jp

[Total Budget for FY1996-FY1998]

16,299,000 Yen (FY1997;8,105,000 Yen)

[Abstract]

Aims to proliferate a small number of primordial germ cells (PGCs) of threatened birds, the embryonic blood was analyzed as a environment of the PGCs, and KAv-1 medium having a pH 8.0 was established. Using this culture medium, the survival rate of PGCs in vitro is surprising improved, and they increased their number at over a hundred times for 7 days incubation on the embryonic feeder cells with growth factors.

[Key Words]

primordial germ cells (PGCs), germ-line chimera, threatened birds, in vitro, pH, cell proliferation.