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E-1.3 Study on genetic relationship between individuals in tropical rainforest.

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[Total Budget for FY1996-1998]

　27,050,000 Yen
　(FY 1998; 9,020,000 Yen)

［Abstract］

　Microsatellite markers were developed from Shorea curtisii using. Analysis of 40 individuals of S.curtisii from natural forest in Malaysia revealed that all SSR loci were polymorphic. Four SSR markers, Shc01, Shc04, Shc07, and Shc09 showed highly polymorphic. We have also compared the genetic diversity between three populations with the different generattions with DBH>5cm, 1cm>DBH>5cm and seedlings of 1 year old. The genetic diversity of seedling population was not high comaring to those of the other generations. The limited number of parent trees, therefore, might be contributed to the next generations. We have also tested the applicability of these SSR primers to other Dipterocarpaceae species using PCR amplification. Since the flanking region sequences of the S.curtisii SSR were well conserved within this family, the SSR primers forS.curtisii can be applied to almost all Dipterocarpaceae species. The average of outcrossing rate in the trees of natural forest was 86.4 % and showed similar tendency of the other dipterocarps species. We also investigated five individuals in logged forest to clarify the genetic diversity and inbreeding rate. The inbreeding rate was quite different in each mother tree and ranged from 21.2 to 85.7 %. with the average of 53.6 %. The inbreeding rate between natural and logged forests was also quite different.

［Key Words］

　Dipterocarpaceae, microsatellites, Shorea curtisii, Genetic diversity, outcrossing rate