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 (651Kb)
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11,010,000 Yen (FY1995 3,223,000 Yen)
Electro-ejaculated deer(Cervus nippon) semen and epididymal spermatozoa collected from hunted Japanese serow(Capriconis crispus) were diluted with the first diluent (NF3-A) and the second diluent (NF3-B) before filling into 0.5ml straws. The straws were then exposed to liquid nitrogen vapor for 10 min. This freezing techniques resulted in approximately 30-40% motile spermatozoa after thawing.
To examine a function of embryonic diapause of Tammar Wallaby bovine embryos were co-cultured with endometrium cell monolayer, which was collected from Tammar Wallaby at embryonic diapause state. Under the conditions of present study, no embryos changed to diapause state.
Ovaries and testis were removed from Japanese serow(Capriconis crispus) shot by gun for an authorized habitat control in Nagano Prefecture. Epididymal sperm were collected and frozen in NF3 and used for in vitro insemination. Nine to 41 oocytes were collected from three femaie serow. The oocytes were cultured in TCM 199 at 38.5℃ for maturation. But the maturation rate was low. Several oocytes were penetrated by sperm but no cleaved. The IVM/IVF system of cattle was adapted for serow with a limited success.
The effects of concentration of glycerol and sugars added in Lake solution were researched for the cryo-preservation of fowl spermatozoa. Seventy % of motile spermatozoa were observed in the composition of 2.5% glycerol, raffinose and torehalose. This result indicates that freezing of fowl spermatozoa above solution will be possible to direct insemination of hen without removing glycerol. It was also demonstrated that the semen of the Japanese copper pheasant was well frozen with the modified Lake solution.
Cryopreservation, Sperm, Oocytes, Pheasant, Deer, Wallaby