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[F-3.2.2 Preservation of Genes of Endangered Wild Animals(Final Report)]

[Contact Person]

Susumu Ishii, Department of Biology, School of Education
Waseda University, Nishi-Waseda 1-6-1, Tokyo 169-50
Phone 81-3-5286-1519, Fax 81-3-3207-9694
E-mail susumu@mn.waseda.ac.jp

[Total Budget for FY1993-1995]

21,151,000 Yen (FY1995 5,854,000Yen)

[Abstract]

　Dispersed cells of inner organs of a Japanese male of the Japanese crested ibis, Nipponia nippon, and those of a Chinese male of the same species were cryo-preserved. The preserved cells of the former individuals were cultured and revealed to proliferate over several generations. Tissue pieces of the former bird were frozen and preserved in liquid nitrogen. To monitor gonadal developmental conditions, we devised a non-invasive method in which sex steroid hormone concentrations in feces are used as the index. This method may be used for selection of individuals for captive breeding of endangered birds. We also developed a method to induce oocyte growth, ovulation and oviposition by hormone administration. By means of the implantation of primordial germ cells, one of us could produce a cimeric gonads of the domestic fowl and the Japanese quail, but no chick of the heterologous species has been produced.

[Key Words]

Cyropreservation, Hepatic cells, Kidney cells, Testicular cells, Japanese crested ibis, Nipponia nippon, Fecal sex steroid hormone