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[E-2.4 Molecular Phylogeny and Genetic Diversity of Dipterocarpaceae in Malaysia]

[Contact Person]

Yoshihiko Tsumura
Bio-resources Technology Division
Forestry and Forest Products Research Institute
Kukizaki, Ibaraki 305 Japan
Phone +81-298-73-3211, Fax +81-298-73-3795
E-mail ytsumu@ffpri.affrc.go.jp

[Total Budget for FY1993-1995]

22,431,000 Yen (FY1995 6,535,000 Yen)

[Abstract]

　A molecular phylogeny of the Dipterocarpaceae subfamily Dipterocarpoideae was constructed using restriction fragment length polymorphisms of polymerase chain reaction amplified specific genes in chloroplast DNA. The phylogenetic trees clearly separate species with two different base chromosome numbers. Our conclusions agree with a phylogeny derived from wood anatomy data analysis, and Symington's and Ashton's taxonomic classifications. We selected five mother trees which are isolated from the other same species trees in Pasoh reserved forest to investigated the outcrossing rate of Neobalanocarpus heimii.. That resulted that the outcrossing rate of this species was quite high, its 1.065(SD=0.212). RAPD data also supported this result. We have investigated the genetic variation of mitochondrial DNA(mtDNA) and RAPD in Hopea species to clarify the genetic diversity and speciation of Dipterocaraceae. The five species could be separated into three groups using mtDNA variation data. This grouping resembles the taxonomic subsections of Hopea species. Hopea species have rather smaller diversities(their overall average is 0.0104±0.0009) than Shorea species(their oveall average is 0.0189±0.0012) in RAPD analysis.

[Key Words]

Dipterocarpaceae, Molecular Phylogent, Genetic Diversity, Evolution