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[A-4.3 Studies on protein changes in UV-irradiated human skin cells]

[Contact Person]

Hirotomo Yamada
Senior Researcher
Department of Industrial Physiology, The Natnl Inst. of
Industrial Health, Ministry of Labor
21-1 Nagao 6 chome, Tama-ku, Kawasaki 214, Japan
Phone +81-44-865-6111, Fax +81-44-865-6116

[Total Budget for FY 1993-FY1995]

18,661,000 Yen (FY1995, 4,193,000 Yen)

[Abstract]

　Specific inhibition of metallothionein (MT) induction by monochromatic UVB lights was studied in human skin cells and HeLa cells When the cells were irradiated with 280-290nm UV lights even at low doses which did not affect total protein synthesis, the induction of MTs by heavy metals and dexamethazone was immediately inhibited. Northern hybridization showed level of Cd-induced MT-mRNA was suppressed markedly after UV irradiation at 280nm. The inhibition of MT synthesis caused by suppression of MT-mRNA deprived the cells of resistance to heavy metals. The result suggests that at least a part of toxic effects of UVB irradiation could result from the inhibition of MT induction.

[Key Words]

UVB Irradiation, Human Skin Cells, Metallothionein, Suppression of mRNA, Heavy Metals