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26,891,000 Yen (FY1995 8,853,000 Yen)
¡¡In a series of studies about the UV effect on the humoral immunity of mice using influenza virus vaccine , we found that the antibody response was not suppressed even by prolonged UV-B irradiation. To examine this more precisely, herein, we used purified Tetanus toxoid and introduced ELISA to determine antibody production. In the early stage, one week postimmunization, the humoral immune response was extensively promoted compared to that in control mice. Herein, the conditions for irradiation were examined.
Humoral immunity, Early promotion, Up-regulation
¡¡Expression of cytokines related inflammation was studied in UV-irradiated skin. IL-1 ¦Â and IL-6 mRNAs which was not detected in normal skin, were progressively expressed in the skin on day 2 after UV-irradiation. ELISA showed that IL-6 protein but not IL-1 ¦Â was produced in the UV-irradiated skin, followed by an increase in its amount circulating in plasma.
Cytokine, IL-6, Acute phase response, Sialic acid
¡¡To determine whether the production of T-cell-derived cytokine(IFN-¦Ã, IL-2 and IL-4) by immune cells from irradiated mice is also suppressed, we examined the production of cytokine by lymph node cells and spleen cells taken from UV-B irradiated, herpes simplex virus type 1(HSV-1)-infected mice. UV-B irradiation prior to HSV-1 infection was found to markedly suppressed IFN-¦Ã but less in IL-2 production compared to that of the non-irradiated control and IL-4 production slightly enhanced by UV-B irradiation.
Cytokines, IFN-¦Ã, IL-2. IL-4, Herpes simplex virus