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[E-2.3.1 Genetic Diversity of Dipterocarpaceae in Tropical Forest]

[Contact Person]

Yoshihiko Tsumura
Bio-resources Technology Division,Forestry and Forest Products
Research Institute, Ministry of Agriculture, Forestry and Fishery
Kukizaki, Ibaraki, 305 Japan
Tel 81-298-73-3211(Ext.444), Fax 81-298-74-3720
E-mail hyoshi@ffpri.affrc.go,jp

[Total Budget for FY1990-1992]

14,564,000 Yen

[Abstract]

　We tried to clarify the genetic diversity of Dipterocarpacea species using a molecular genetic maker. Plylogenetical relationship between 21 species of seven genera was investigated using RFLP(Restriction Fragment Length Polymorphism) of chloroplast DNA. When phylogenetic tree was constructed based on these data, the result was supported Ashton's classification based on morphological trait. Mating system of Neobalanocarpus heimii was also surveyed using gene makers, namely, allozyme and RAPD(Random Amplified Polymorphic DNA). In this case, five isozyme loci were used for estimation of outcrossing rate. This result was 1.065(SD; .212), thus, this species was mostly a outcrossing species. The RAPD data also supported the result.

[Key Words]

Diperocarpacea, Tropical Forest, Molecular Phylogeny, Mating System, Genetic Diversity