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34,055,000 Yen
A large scale axenic culture tank was used in order to understand the causative relationship between the growth of phytoplankton and the variation of CO2 concentration in atmosphere. Variations of Organic carbon and inorganic carbon in sea water and absorption of atmospheric CO2 into sea water had been measured in the tank in detail. About 17% of assimilated inorganic carbon was excreted from the cells as dissolved organic car-bon, which is very important for the production of DOC in the surface layer of the ocean. It was found that the growth of C. antiqua was limited by available [H2CO3*] instead of [HCO3-].
Marine microcosm, ocean carbon cycle, Marine phytoplankton, carbon fixation