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 (238Kb)
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28,899,000 Yen
Our objectives are to investigate the expression of newly induced protein in UVB exposed mice or cultured cells, to clarify modulation of mast cell differentiation from UVB exposed bone marrow cells and to establish a new mutation assay system. The results obtained are as follows: 1) The methods to detect the expression of a variety of new synthesized proteins in UVB irradiated cells were determined. 2) Although the growth of mast cells was suppressed by UVB irradiation the expression of IgE receptor on mast cells was increased when compared to those from control. 3) Retroviral vector LTK-15 constructed with HSV-TK and neo genes was established for detecting the mutation caused by UVB. 4) We have established a skin cancer model by using hairless mice. The model could be utilized to study mechanisms of gene expression by UVB irradiation and those of stress-mediated protein synthesis. 5) It was shown that the effects of active oxygen species, which are produced by UV irradiation in a single exposure study, can be suppressed if MT or GSH levels can be increased in the tissues. On the other hand, in a repeated-exposure study, a decrease in endogenous GSH and an increase in MDA do not occur, suggesting the presence of an adaptation of skin tissues to UVB irradiation.
Skin Cancer, Metallothionein, Mutation Gene, Mast Cell